Petri dish methods - actinomycetes bacteria nutrient media

Petri dish methods - actinomycetes/bacteria (nutrient media) 

Petri dish tests can demonstrate that a plastic part can inhibit or support the growth of particular organisms. Nutrient media is placed in a petri dish and allowed to gel. The test sample is placed on the media previously inoculated with bacteria or actinomycetes. After incubation, the microbiologist measures the zone of inhibition or the area surrounding the plastic where growth of the organism was inhibited. This inhibited zone is due to 

Bacteria such as Staphylococcus aureus, Klebsiella pneumonia, as well as Actinomycetes such as Streptoverticillium can be used. The test samples are evaluated for microbial stain or growth of cellular colonies in the 

This zone of biocide diffusion is affected by the following  

Solubility of the biocide in the polymer matrix Biocide concentration Biological challenge 

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