Peroxidases solvent composition

Enzyme selectivity is usually limited because it depends on the interaction between the substrate and hydrophobic and hydrophilic amino acid residues at the active site, but here the degree of substrate immobilization is generally low. After the electron transfer process has occurred, the substrate is transformed into a radical compound that diffuses to the bulk of the solution and evolves according to its chemical properties, generally independently of the enzyme. This implies that the peroxidases rule the yield and the rate of radical formation but, once the latter species has been formed, the product composition and the stereoselectivity of the reaction are essentially dependent on the radical chemical structure and, to some extent, on the solvent and temperature of the reaction. 

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