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Peptides synthesis genetic engineering methods

The ability to synthesize chemically short sequences of single-stranded DNA (oligonucleotides) is an essential part of many aspects of genetic engineering. The method most frequently employed is that of solid-phase synthesis, where the basic philosophy is the same as that in solid-phase peptide synthesis (see Section 13.6.3). In other words, the growing nucleic acid is attached to a suitable solid support, protected nucleotides are supplied in the appropriate sequence, and each addition is followed by repeated coupling and deprotection cycles. [Pg.566]


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See also in sourсe #XX -- [ Pg.241 , Pg.242 , Pg.243 , Pg.244 , Pg.245 ]

See also in sourсe #XX -- [ Pg.241 , Pg.242 , Pg.243 , Pg.244 , Pg.245 ]




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