Peptides solid-phase sequencing

One step further, namely to monomeric sequence-defined polymeric vehicles, leads to the synthetic strategy based on standard peptide solid phase chemistry. 

R. Aebersold, G.D. Pipes, R.E. Wettenhall, H. Nika, and L.E. Hood. 1990. Covalent attachment of peptides for high sensitivity solid-phase sequence analysis Ana/. Biochem. 187 56-65. (PubMed)... 

Solid-phase sequencer, an apparatus for peptide/protein sequence analysis. Automated primary structure determination of peptides and proteins using the solid-phase sequencing method is based on cova-... 

The design and construction of the solid-phase peptide sequencer have been discussed in detail (Laursen et ai, 1975). Basically, the instrument consists of a thermostatted reaction column, which holds the resin-bound peptide, and a series of pumps and valves that deliver reagents and solvents to the column. After each cycle, the liberated thiazolinones are collected in a fraction collector. The instrument is fully automatic and completes a cycle in 60-90 min, depending on how it is programmed. Solid-phase sequencers based on the design of Laursen (1971 Laursen et a/., 1975) are available from Sequemat, Inc. (Watertown, Massachusetts), LKB Instruments (Rockville, Maryland), and Rank-Hilger (United Kingdom). 

Solid-phase sequencing has great potential for microsequencing, especially of hydrophobic polypeptides. Since the peptide is covalently bound, me-... 

In the future, the technique of solid-phase sequencing will be most aided by improved peptide coupling methods and a reduction in the side reactions of the Edman degradation. 

Chapter 6 Solid-Phase Sequencing of Peptides and Proteins... 

Since Laursen s first report of solid-phase sequencing using a polymer-bound peptide, many other polymeric supports have been developed for this purpose. These are listed in Table 8-1. These polymeric supports are of three types styrene-based polymers, silylated aminoalkyl glass, and polyamides. 

VII. SOLID-PHASE SEQUENCING OF PEPTIDES FROM THE CARBOXYL TERMINUS... 

Memfield successfully automated all the steps m solid phase peptide synthesis and computer controlled equipment is now commercially available to perform this synthesis Using an early version of his peptide synthesizer m collaboration with coworker Bemd Gutte Memfield reported the synthesis of the enzyme ribonuclease m 1969 It took them only SIX weeks to perform the 369 reactions and 11 391 steps necessary to assemble the sequence of 124 ammo acids of ribonuclease... 

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