Peptides, Proteins, and Glycoproteins

In this chapter, we describe the mapping, ladder, and fragmentation approaches for the structural analysis of peptides, proteins, and glycoproteins, in some cases using 

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Native chemical ligation (NCL) has shown great utility and is a proven method for the preparation of peptides, cyclic peptides, proteins, and glycoproteins via synthetic or semi-synthetic pathways [1-5]. The concept of NCL dates back to the pioneering work of Wieland et al. [6], but it was not until 1994 that this method gained widespread attention when Kent et al. reported its application in the synthesis of interleukin-8, a cytokine responsible for the proliferation of B cells during immune response [7]. 

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