Peptides glycosylation process

Rituximab is a recombinant mouse/human chimeric monoclonal antibody whose in vitro activity varies with the number of terminal galactose moieties glycosylated to the peptide backbone at residue asparagine 301 [8]. The ability to monitor the levels of each discrete species present would allow the manufacturing process to... 

Figure 3. Schematic representation of the PGII, PGI, PGC [13] and PGE proteins from A. niger, indicating the putative processing sites for the signal peptide ( ) and the mono- and dibasic processing site for the propeptide ( ). The position of introns (lA, IB and IC) are indicated ( [) and variation of amino acids number is shown in different parts of protein. The putative N-glycosylation sites are marked ( ).

Lack of the glycosylation of the common precursor of ACTH-jSLPH resulted in its rapid degradation, and formation of atypically processed peptides which were, however, still secreted. The decreased stability against proteases in vivo was also reflected by its lowered proteolytic stability in vitro the isolated, nonglycosylated precursor was degraded much more readily by protease than the glycosylated counterpart.439-441... 

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