Peptides field strength optimization

The ability to inhibit wall interaction is demonstrated in Fig. 5. The complete tryptic digest of cytochrome c is analyzed at pH 4.0, with optimization accomplished by modifying the ionic strength of the buffer and separation field strength. A dramatic improvement in the separation is achieved by increasing the ionic strength. From these data, the best separation conditions can easily be identified, and the complex mixture spiked with the isolated peptides to identify them within the mixture. 

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