Peptide encoding strands

A four-base overlap between dihydrofolate reductase and thymidylate synthase has been found201 in the DNA of phage T4. A transposable DNA insertion sequence (see Section D,5) in E. coli encodes two genes, one of which is contained within the other and which is transcribed from the opposite strand of DNA.202 The double-stranded RNA of a reovirus produces two peptides from the same sequence using two different AUG initiation codons in different reading frames 203... 

The DNA strands were first reported by Brenner and co-workers to define the sequence of a peptide constructed on a solid support. Upon completion of the synthesis, an on-bead assay was performed. Whereas each bioactive peptide was defined by a unique DNA sequence, the decoding process simply involved amplification of the code by the polymerase chain reaction followed by sequencing. This technique marked the beginning of the tagging method for encoded split synthesis. Sequenceable peptide strands are an alternative to DNA encoding. The code is read by HPLC of the Edmon degradation phenylthiohydantoin amino acid derivatives, a well-developed microsequencing method. 

Figure 6 Schematic representation of CPMV (a) RNAs, (b) coat protein subnnit, and (c) structnre (a) The genomic material of CPMV is composed of two nniqne strands of RNA. RNA-1 encodes for the viral replication machinery. RNA-2 encodes for the viral capsid proteins. Each strand of RNA is individually packaged into its own viral capsid, (b) Ribbon diagram illustrating the interaction between the L coat protein (cyan and green) and the S coat protein (blue), (c) Illustration of the ovCTall structure of CPMV with a genetically engineered peptide displayed (depicted in red) on the surface of the coat protein owing to the insertion of its sequence at the loop of the S subunit as highlighted by the red arrow in (b). (Reproduced with permission from Ref. 32. Annual Reviews,...

---