Peptide Bioactive peptides Cationic

The simultaneous separation and recovery of acidic and basic bioactive peptides by employing electrodialysis with ultrafiltration membranes has also been investigated recently [30]. This work aims at demonstrate the feasibility of separating peptides from a beta-lactoglobulin hydrolysate, using an ultrafiltration membrane stacked in an electrodialysis cell, and a study of the effect of pH on the migration of basic/ cationic and acid/anionic peptides in the electrodialysis configuration. 

Because of their well-recognized physicochemical properties (relatively high hydrophobicity, cationic character, and short length) reversed-phase, size-exclusion, and cation exchange chromatographies are particularly appropriate to purify bioactive peptides from the immune system of invertebrates. The sensitivity of HPLC, MS, Edman degradation, and liquid growth inhibition assays allow one to use from narrow (e.g., 2.1-mm internal diameter) down to micro-or nano-columns. 

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