Peptide backbone cleavage

Partial Reduction of Multiple-Cystine Peptides and Peptide Backbone Cleavage by... 

A particular form of CNB is (2-nitrophenyl)glycine (Npg). This artificial amino acid (Npg, Fig. 3.2-ID) was successfully incorporated into ion channels like the nicotinic acetylcholine receptor [22] by nonsense suppression, a technique developed by Peter Schultz and coworkers [23-26]. Irradiation (4 h, >360 nm) of proteins containing Npg led to peptide backbone cleavage in Xenopus oocytes [22],... 

Neutral Loss-Driven MS This triggers additional fragmentation of the dominant and specified NL-associated peak observed in the MS/MS scan. It results in the generation of more fragment ions for accurate peptide sequence determination. MS spectra are helpful when peptide backbone cleavage by MS/MS is inefficient. 

Scheme 17.28. Peptide folding can explain the role of the nonmobile proton in peptide backbone cleavage.

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