Pentyl-pyridoxamine

The identification of the key features for IsoK/LG scavenging led to the synthesis of other related phenolic amines that also potently scavenge IsoK/LG in vitro such as pentyl-pyridoxamine (Davies et al., 2006). Lipophilic scavengers such as salicylamine and pentyl-pyridoxamine localize to the membranes where... 

IsoK/LG form and should therefore be more effective in vivo than hydrophilic compounds such as pyridoxamine. The greater efficacy of salicylamine and pentyl-pyridoxamine compared to pyridoxamine was borne out in studies where platelets were treated with individual scavengers and then activated with arachidonic acid to make LGs. While all three scavengers significantly inhibited the formation of LG protein adducts, salicylamine was the most potent followed by pentyl-pyridoxamine and then pyridoxamine (Davies et ah, 2006). 

Fig. 2.7 Lipophilic IsoK/LG scavengers protect against cytotoxicity induced by oxidative stress. HepG2 cells were incubated with vehicle, pyridoxamine, pentyl-pyridoxamine, or salicylamine for 30 min prior to treatment with various concentrations of hydrogen peroxide (Davies et al., 2006). Viability was determined by detection of ATP using ATPlite luminescence assay and percent viability calculated relative to untreated cells (Mean SEM n = 8)...

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