Pentaerythritol tetranitrate reductase

French CE, S Nicklin, NC Bruce (1996) Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB 2. J Bacteriol 178 6623-6627. 

Basran J, RJ Harris, MJ Sutcliffe, NS Scrutton (2003) H-tuneling in the multiple H-transfers of the catalytic cycle of morphinone reductase and in the reductive half-reaction of the homologous pentaerythritol tetranitrate reductase. J Biol Chem 278 43973-43982. 

An unusual reaction was been observed in the reaction of old yellow enzyme with a,(3-unsat-urated ketones. A dismutation took place under aerobic or anaerobic conditions, with the formation from cyclohex-l-keto-2-ene of the corresponding phenol and cyclohexanone, and an analogous reaction from representative cyclodec-3-keto-4-enes—putatively by hydride-ion transfer (Vaz et al. 1995). Reduction of the double bond in a,p-unsaturated ketones has been observed, and the enone reductases from Saccharomyces cerevisiae have been purified and characterized. They are able to carry out reduction of the C=C bonds in aliphatic aldehydes and ketones, and ring double bonds in cyclohexenones (Wanner and Tressel 1998). Reductions of steroid l,4-diene-3-ones can be mediated by the related old yellow enzyme and pentaerythritol tetranitrate reductase, for example, androsta-A -3,17-dione to androsta-A -3,17-dione (Vaz etal. 1995) and prednisone to pregna-A -17a, 20-diol-3,ll,20-trione (Barna et al. 2001) respectively. 

Khan H, T Barna, RJ Harris, NC Bruce, I Barsukov, AW Munro, PCE Moody, NS Scrutton (2004) Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. J Biol Chem 279 30563-30572. 

French, C. E., Rosser, S. J., Davies, G. J., Nicklin, S., and Bruce, N. C., 1999, Biodegradation of explosives by transgenic plants expressing pentaerythritol tetranitrate reductase, iVort. Biotechnol. 17 491-494. 

French, C.E., S. Nicklin, and N.C. Bruce. 1998. Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerythritol tetranitrate reductase. Appl. Environ. Microbiol. 64 2864—2868. 

H-Tuiweling in Morphinone Reductase and Pentaerythritol Tetranitrate Reductase 1347... 

Mueller, N.J., Stueckler, C., Hauer, B., Baudendistel, N., Housden, H., Bruce, N. C., and Faber, K. (2010) The substrate spectra of pentaerythritol tetranitrate reductase, morphinone reductase, N-ethylmaleimide reductase and estrogen-binding protein in the asymmetric bioreduction of activated alkenes. Adv. Synth. Catal., 352, 387-394. 

Fryszkowska, A., Toogood, H., Sakuma, M., Gardiner, J.M., Stephens, G.M., and Scrutton, N.S. (2009) Asymmetric reduction of activated alkenes by pentaerythritol tetranitrate reductase specificity and control of stereochemical outcome by reaction optimisation. Adv. Synth. Catal., 351, 2976-2990. 

Pentaerythritol tetranitrate reductase (PETNR) Enterobacter cloacae 1996 [10]... 

Khan, H., Bama, T, Bmce, N.C., Munro, A.W., Leys, D., and Scmtton, N.S. (2005) Proton transfer in the oxidative half-reachon of pentaerythritol tetranitrate reductase. FEBS/., 272, 4660-4671. 

Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries. ChemBioChem, 11, 2433-2447. 

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