PEL Pseudomonas fluorescens lipase

PEG-PCL Pseudomonas cepacia bound to polyethylene glycol (PEG) PEL Pseudomonas fluorescens lipase... [Pg.453]

Many lipases, such as porcine pancreatic lipase (PPL), Candida cylindracea lipase (CCL), Chromobacterium viscosum lipase (CVL), Candida antarctica lipase (CAL), Candida antarctica lipase B (CALB, Novozyme-435), polyethylene glycol (PEG)-modified Pseudomonas fluorescens lipase (PEL), Pseudomonas cepacia lipase (PsCL),... [Pg.422]

Amongst the lipases, the pig pancreatic lipase (PPL), the yeast lipase from Candida cylindracea (rugosa) (CCL), and the bacteria lipases from Pseudomonas fluorescens (cepecia) (PEL) and other unclassified Pseudomonas species (PSL) have been most widely used. The experimental methods are very straightforward and little different in their execution from conventional chemical reactions. Hydrolysis reactions are conducted on the soluble lipase in buffered aqueous solutions, commonly in the presence of an organic cosolvent. In organic media the enzyme is added as a powder or in an immobilized form and the resulting suspension stirred or (better) shaken at approximately 40 °C. The enzyme is removed by filtration. [Pg.377]