Pectin sulphate

Figure 5. Analytical isoelectric focusing. Ultrathin layers (0.4 nun) of polyacrylamide with ampholytes pH 2-11 were used. Samples of 10 pg of protein in 10 pi of 1 % glycine were applied. A.- Silver staining. B.- Stain for activity on overlays containing pectin in tris/HCl buffer at pH 8.0 with CaClj M.- Broad pi Calibration Kit protein (Pharmacia), samples of 5 pg of protein were applied. 1.-Ammonium sulphate precipitated proteins from cultures on pectin. 2.- Fractions with PNL activity eluted from the Superdex 75HR1030 column. 3.- Purified PNL.

Neutral detergent fibre (NDF) Sodium lauryl sulphate, EDTA, pH 7.0 Boil for 1 h Herbage cell wall minus pectins... 

Under salts, el cetera, are included malic acid and gum, nitrogenous "subatanoes, fatty matters, aromatic substances, coloring matters, essential oil, chlorophyle, malamide, oxalate and phosphate of lime, phosphata of magnesia, chloride of, ammonium, silicate, nitrate, sulphate, and oxalate of potassa oxalate of soda, chlorides of sodium and potassium, peetates and pectinates of... 

Heparin causes a reduction in mitotic index and a decrease in tumour volume for Ehrlich ascites tumours in mice . Polyethylene sulphonates having molecular weights between 15,000 and 35,000, also have antineo-plastic activity in mice against ascites tumours, carcinomas and leukaemias . Heparin has been found to reduce markedly the number of pulmonary metastases in rats receiving intravenous Walker 256 carcinoma cells . Evans blue in the presence of a sulphated pectin entered tumour cells but not normal tissue cells s. The anti-immunochemical action of heparin has been used successfully for tumour heterografts Ass. 

Thus the proteins have carboxyl, amino, guanidino, imida ol groups, substances such as gum arabic and pectinic acid have carboxyl groups, agar ester sulphate groups, nucleates phosphate groups as carriers of the electric charge. 

For this investigation a sulphate colloid (Na agar), a carboxyl colloid (Na pectinate) and three phosphate colloids (Na yeast nucleate, purified egg lecithin, and a soya bean phosphatide fraction soluble in alcohol) were used. 

The experimental results show that a simultaneous resemblance of ephedrine and Ca on the one side and of acetylcholine and K on the other side is present neither in sulphate colloids (agar) nor its substitute (Si02), nor in carboxyl colloids (pectinate) nor in the phosphate colloid nucleate but only in phosphatides. 

Conditions for the maximum yield of extracellular a-amylase from Bac-teroides amylophilus have been reported. Isoelectric focusing and polyacrylamide gel electrophoresis demonstrated the presence of six isoenzymes one of these was purified by ion-exchange chromatography and gel filtration. This enzyme (pH optimum 6.3, temperature optimum 43 °C, pH stability range 5.8—7.5, p7 4.6, mol. wt. 9.2 x 10 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis) was inhibited by cyclo-hexa and hepta-amyloses, phenyl a-D-glucopyranoside, and Hg + whereas Ca + and Co were strong activators. The relative rates of hydrolysis of amylose, soluble starch, amylo-pectin, and dextrin were 100, 97, 92, and 60%, respectively (Am values 18.2, 18.7, 18.2, and 16.7 [xmol D-glucosidic bonds 1, respectively). 

---