Pectin methylesterase structure

These protruding structures may form catalytic sites or binding sites for small molecules. Remarkably, some polysaccharide modification enzymes of pathogenic bacteria are similar to enzymes of their host cells. For example, during cell development, pectin, a principal component in the primary cell wall of plants, is modified by its own pectin methylesterases that have /1-solenoid structures (Johansson et al, 2002) and in this respect, resemble pectin lyases secreted by bacteria to break down these structures (Lietzke et al., 1996). 

Jenkins, J., Mayans, O., Smith, D., Worboys, K., and Pickersgill, R. W. (2001). Three-dimensional structure of Erminia chrysanthemi pectin methylesterase reveals a novel esterase active site./. Mol. Biol. 305, 951-960. 

Johansson, K., El-Ahmad, M., Friemann, R., Jomvall, H., Markovic, O., and Eklund, H. (2002). Crystal structure of plant pectin methylesterase. FEBS Lett. 514, 243-249. 

For bUayer formation in emulsions in a first set of experiment, commercially available pectin samples were used [51 ]. To get a deeper understanding of the impact of molecular structure of the pectin on the functionality of the bilayer-stabilised emulsions, pectin samples with well-defined degree of methoxylation and distribution of these groups were produced on the lab scale. Enzymatic demethoxylation was performed in a similar way using either a commercially available fungal pectin methyl esterase or plant-derived pectin methylesterase, which was isolated from orange peel as described in Stoll et al. [52]. Commercially available high... 

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