PEAK ASSIGNMENT AND RECOGNITION

By definition, all interpretive methods of optimization require knowledge of the capacity factors of all individual solutes. This is the fundamental difference between the simultaneous and sequential methods of optimization (sections 5.2 and 5.3, respectively) and the interpretive methods of section 5.5. Moreover, in the specific cases in which only a limited number of components is of interest or in which weighting factors are assigned to the individual solutes (see section 4.6.1) it is also necessary to recognize the individual peaks (at least the relevant ones) in each chromatogram. In section 5.5 we have tacitly assumed that it would be possible to obtain the retention data (capacity factors) of all the individual solutes at each experimental location. 

The problem of recognizing the individual solutes in the chromatograms during the 

Selection of interpretive methods applied for selectivity optimization in chromatography. 

In this section different ways to measure the required data will be discussed. 

Model Application (compositions refer to mobile phase unless stated otherwise) 

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