Patch-clamp fluorescence microscopy

Probing Conformational Changes of Gramicidin Ion Channels by Single-Molecule Patch-Clamp Fluorescence Microscopy. 

Ryttsen, R, Farre, C., Brennan, C., Weber, S.G., Nolkrantz, K., Jardemark, K., Chiu, D.T., Orwar, O., 2000. Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy. 

Despite the predominant use of fluorescence and/or patch clamp techniques in single cell measurements, there has been a steady increase in the demand for new electroanalytical tools applicable to single cell studies [4]. Traditionally, such methods have been confined to the development and production of hand crafted sensors including the aforementioned glass capillaries [1-3] for patch clamping, as well as conical microelectrodes for scanning electrochemical microscopy (SECM) [5, 6] and carbon fiber microelectrodes to measure for example, the release of neurotransmitter from single neurons [7, 8]. 

Live-Cell Microscopy for GFP Fluorescence Detection and Taipeting for Whole-Cell Patch Clamp Electrophysiology... 

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