Partner of Numb

Miranda (Mir Shen et al 1997, Ikeshima-Kataoka et al 1997), Staufen (Li et al 1997, Broadus et al 1998, Schuldt et al 1998) and Partner of Numb (Pon Lu et al 1998), act as a link between the apically localized Insc and the basally localized cell fate determinants. These adaptors act downstream of insc and are also asymmetrically localised, similar to the cell fate determinants they help to localize, in an /Arc-dependent manner. 

Lu B, Rothenberg M, Jan LY, Jan YN 1998 Partner of Numb, a novel protein that colocalises with Numb during mitosis, directs Numb asymmetric localisation in Drosophila neural and muscle progenitors. Cell 95 225-235... 

Chia The only molecule that has been looked at in living cells is Partner of Numb (PON), which has been looked at by Kuh-Nung Jah s lab. They see with GFP—PON that it is cytoplasmic during interphase and goes to the cortex in early prophase. Then the molecules appear to migrate to the basal cortex later on in prophase and are anchored there. 

FIG. 1. Localization of key proteins involved in the neuroblast asymmetric cell division. During late interphase a complex of proteins including Insc, Baz (and Pins) are localized to the apical cell cortex. This complex acts to mediate the basal cortical localization of the cell fate determinants Numb (and its partner Pon), Pros (and its partner Miranda) and pros RNA (and its partner Staufen) during mitosis. During interphase, Numb is cytoplasmic and Pros is localized to the apical cortex. 

A very substantial majority (68%) of these users reported that marijuana disdncdy enhanced their sexuality. Even more interesting, 50% of the women and 39% of the men revealed that it actually excited their initial interest, sharpened their sexual desire—again, only when smoked with a desirable sexual partner. If smoked with someone deemed distasteful, they said, marijuana accentuates the feeling of distaste. Sex actually became repugnant. "It s not like alcohol, which often numbs a person to the unpleasant aspects of his partner," Dr. Goode commented. 

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