Partial identity

Bumble bee venom contains also a phospholipase A2 with partial identity to bee venom phospholipase Aj and a protease, but no melittin. Instead there are several small peptides called bombolitins [9]. There is limited cross-reactivity between honey bee and bumblebee venoms [2]. 

The same principle is the fundament of the WARREN-AVERBACH method (cf. Sect. 8.2.5.5) for the separation of size and distortion of structural entities. Thus die mathematics is partially identical. 

Figure 7.7 Double diffusion in gels showing reactions of identity, non-identity and partial identity.

The anti-S. faecalis antiserum reacted with Lac-BSA amd the glycan as shown in the bottom panels of Figure 8. The amti-lac antibodies from this serum also reacted with these two immunogens yielding a similar pattern as the unfractionated amtiserum. It should be noted that the amtiserum amd amtibody preparations exhibited partial identity with these two antigens. The anti-gal antibodies also reacted with Lac-BSA amd the glycan but the precipitin bamds were quite different. 

Perhaps the clearest demonstration of HDL heterogeneity was that by Suenram et al. (S61). When specific antisera to apolipoproteins A-I, A-II, B, C-III, D, E, and F were set up in double diffusion analyses against HDL, reactions of nonidentity were observed between each possible combination of these antisera. The only exception was a reaction of partial identity between antisera to apoA-I and apoA-II, indicating two types of apoA-contain-ing lipoproteins, a major class containing both proteins and a minor one containing only apoA-I. The other apolipoproteins in HDL therefore appear each to be largely peculiar to their own lipoprotein particles, and the nomenclature LpC, LpD, etc., has been used by Alaupovic and co-workers to describe lipoproteins characterized by a single apolipoprotein class (A5-A7). 

Figure 6-5. Ouchterlony double immunodiffu- gives information about whether the antigens sion. Antibody is loaded into the central well, are the same (left), different (middle) or whether and antigens into the surrounding peripheral there is partial identity (right), wells. The pattern of precipitin lines produced...

If only partial identity exists between the protein in the unknown samples and that in the reference solution, errors in quantitation... 

Figure 5.8. Double-diffusion test of antigen identity (a) identity, (b) nonidentity, and (c) partial identity.

Figure 9-4 Double immunodiffusion in two dimensions by the Ouchterlony technique. A, Reaction of identity B, reaction of nonidentity C, reaction of partial identity D, scheme for spur formation. Ag, Antigen Ab, antibody.

DNA molecules with similar or partially identical sequences. Sometimes from two unmanipulated species, at other times a gene has been modified and reintroduced. 

JWB and the mutant enzyme of tabacum Su/su, whereas only partial identity between these two enzymes and the enzyme of Spinacia oleracea is observed (Fig. 1). Determination of the maximal binding of antibodies onto the enzymes to be compared out of the homologous and non-homologous monospecific antisera according to the methods of Heidelber-ger and Kendall (4,5) have led to the result that in the region of... 

It appears that g74 and g75 each represent a set of allotypic determinants, present on both the Fab and Fc fragments. For example, in an Ouchterlony test with anti-g74 antiserum either fragment, Fab or Fc, forms a precipitin band which shows only partial identity with intact sIgA i.e., each fragment reacts but is antigenically deficient as compared to the intact molecule. Also, the g74 specificities on Fab and Fc are antigenically distinct from one another. Similar results were obtained with anti-g75 antisera. Specificities controlled by the /locus have not been localized to fragments Fab or Fc because of the resistance of such IgA molecules to papain (111,112). 

Figure 4.10. Addition of two, reversible Carnot cycles with partially identical adiabat. The work contributions Wij and Wji have opposite signs and neutralize each other therefore, the composite process corresponds to a cyclic process following an outer contour line.

After double diffusion according to Ouchterlony of spinach and maize CF or spinach and maize thylakoids against an antiserum against spinach CF (150) or against maize CF (141), the pattern of only partial identity was seen (spurs). By absorption of the respective antisera with heterologous thylakoids we removed all crossreacting ab and thus produced sera, where only the species-specific non-crossreacting ab had remained (Pucheu,Berzborn, in preparation). 

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