Parameters Affecting Retention

Capacity parameters are not often used as primary optimization parameters in chromatography. Therefore, they are only included in table 3.10 in those cases in which they are used with some frequency. It should be noted, however, that changing one of the capacity factors usually involves the use of a completely different column and is therefore unattractive. Although changing the capacity parameters affects retention in an essentially predictable way, changing the column (packing material, film thickness, etc.) may give rise to unexpected second order phenomena. This is a second reason for which capacity parameters should not be recommended as primary optimization parameters. 

General Parameters Affecting Retention Eluent Flow Rate... 

Experimental Parameters Affecting Retention when Applying Suppressor... 

In addition to the type of eluent, its concentration is one of the most important parameters affecting retention. Closely connected is the pH value of the mobile phase as a function of the eluent concentration. When a carbonate/bicarbonate mixture is selected as the eluent, parameters such as ionic strength and pH value cannot be considered separately, as the selectivity changes by varying the concentration ratio of the two compounds. 

---