Paracoccus denitrifi cans

Carr, G. J., and Ferguson, S. J. (1990). The nitric oxide reductase of Paracoccus denitrifi cans. Biochem.. 269, 423-429. 

In the case of cytochrome cdi (Cyt cdi) found in many facultative, anaerobic denitrifying bacteria, the SERRS spectroscopy has been used to obtain preliminary heme structure/environment comparisons between cyt cdx from two bacteria sources. Pseudomonas and Paracoccus. The difficulty encountered in preparing sufficient quantities of cyt cdi, can be thus solved by the low product consuming SERRS method. By exciting selectively with the 514.5 nm and 460 nm lines of an Ar laser, it is possible to produce successively an enhancement of RR scattering of reduced heme c, see Fig. 34, and heme di, see Fig. 35, in the protein complexes (3 x 10 M) adsorbed on Ag at —0.6 V vs. SCE. A comparison between the SERRS spectra of cdi proteins from the bacteria suggests differences in the heme chromophore and peripheral substituents. 

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