Paracetamol calibration curve

Analysis of paracetamol tablets using a calibration curve... 

Calibration curve for the determination of paracetamol in tablets obtained from HPLC analysis of calibration standards. 

Carefully remove 2 ml of die supernatant liquid from each of the centrifuge tubes, add to the appropriate 30-ml tube, and allow to stand for 2 to 3 minutes. To each of the tubes add, dropwise, 2 ml of a 15% solution of ammonium sulphamate to remove excess nihous acid (vigorous frodiing occurs), followed by 2 ml of 6M sodium hydroxide mix for a few seconds to remove any gas bubbles, and measure the intensity of the yellow solutions at 450 nm against the plasma blank. Calculate the concentration of paracetamol in the sample by reference to a calibration curve prepared from die values for die standard solutions. Duplicate analyses of samples and standards should be carried out. 

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