Panning technique cell culture

Tlaskalova-Hogenova et al. demonstrated the usefulness of affinity cell chromatography to isolate T- and B-lymphocytes from human tissues. These authors describe comparative studies on three popular approaches to the isolation of lymphocyte subpopulations, namely nylon wool columns, immunoaffinity cell panning (a batch technique using antibodies immobilized to the bottom of culture dishes), and immunoaffinity using anti-human immunoglobulins attached to Sephron (hydroxyethyl-methacrylate) or Sepharose supports. These studies clearly indicate that the selectiveness of immobilized antibodies was superior for isolating defined subpopulations of cells. 

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