Ovomucoid antibody

FIGURE 16.1 Reactivity of anti-intact ovomucoid polyclonal antibody to heat-treated ovomucoid. Ovomucoid was heat-treated at 50, 100, and 121 C for 20 min, then was assayed by sandwich ELISA using anti-intact ovomucoid antibody. 

The histogram of the interface areas observed in a sample of 75 protein-protein complexes analyzed by Lo Conte etal. (1999) is shown in Fig. 8. In this sample, the majority of the complexes (70%) have interfaces in the range 1200-2000 A. This range includes 19 of 24 protease-inhibitor complexes, 18 of 19 antigen-antibody complexes, and 15 of 32 other types of protein-protein complexes in the sample analyzed by Lo Conte etal. (1999), who define a standard size interface as having = 1600 400 A. General characteristics of such interfaces are listed in Table IV two typical examples are shown in Fig. 6 the chymotrypsin-ovomucoid inhibitor and the FvD1.3-Fv E5.2 interfaces. A standard size interface... 

HiroseJ, KitabatakeN, KimuraA, NaritaH (2004). Recognition of native and/or thermally induced denatured forms of the major food allergen, ovomucoid, by human IgE and mouse monoclonal IgG antibodies. Biosci. Biotechnol Biochem., 68 2490-2497. 

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