Ovine submaxillary gland mucoprotein

The reason for the different values obtained is not readily discernible except for the extremely high values (45 moles/10 gm) of Lieflander and Wacker (1962) which must be due to the high pH used. It may be that the lower pH used by Bello in his determinations (Bello, 1960 Bello and Bello, 1963) is the reason that his values are particularly low (0.5-1.5 moles/10 gm). It is of interest that Graham et al. (1963) could observe no splitting of the ester links in ovine submaxillary gland mucoprotein during treatment with 1 M hydroxylamine at pH 8 and 37°C. Hydroxyamidation of simple esters occurs most readily only at pH values greater than 9 (Botvinik et al., 1962). 

Graham, E. R. B., and Gottschalk, A., 1960, Studies on mucoproteins. I. The structure of the prosthetic group of ovine submaxillary gland mucoprotein, Biochim. Biophys. Acta 38 513. 

Gottschalk, A., and Thomas, M.A.W., 1961, Studies on mucoproteins. V. The significance of N-acetylneuraminic acid for the viscosity of ovine submaxillary gland glycoprotein, Biochim. Biophys. Acta 46 91-98. 

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