Overload Due to Excess Sample Volume

An expression for the maximum charge that can be placed on a column without impairing resolution has already been derived, but the approach, when dealing with an overloaded column for preparative purpose, will be quite different. For preparative purposes the phase system is chosen to provide the maximum separation of the solute of interest from its nearest neighbor. It should be pointed out that the separation may, but probably will not, involve the closest eluting pair in the mixture. Consequently, the maximum resolving power of the column will not be required for the purpose of separation and the excess resolution of the solute of interest from its nearest neighbor can be used to increase the column load. 

Marcel Dekker, In( 270 Madison Avenue, New York, New York 1001 

half the peak width at the base is approximately twice the peak standard deviation, and thus, assuming the two peaks have around the same efficiency (n), 

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