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Ovation biotin RNA amplification and labeling system

Fig. 2. (A) Agarose gel electrophoresis of total RNA (500ng/lane) extracted from four different regions (1-4) of the neural tube of E9.5 CDl mouse embryos using the column protocol detailed in section 3.3. The arrows point to the characteristic 28s and 18s rRNA bands. For intact total RNA, the 28s band should be approximately double the intensity of the 18s band see Notes 1-11). (B) Agarose gel electrophoresis of the biotin labeled extracts generated by the Ovation Biotin RNA Amplification and Labelling System (Enzo) from... Fig. 2. (A) Agarose gel electrophoresis of total RNA (500ng/lane) extracted from four different regions (1-4) of the neural tube of E9.5 CDl mouse embryos using the column protocol detailed in section 3.3. The arrows point to the characteristic 28s and 18s rRNA bands. For intact total RNA, the 28s band should be approximately double the intensity of the 18s band see Notes 1-11). (B) Agarose gel electrophoresis of the biotin labeled extracts generated by the Ovation Biotin RNA Amplification and Labelling System (Enzo) from...
Labeled Extract Synthesis Ovation Biotin RNA Amplification and Labeling System (for lOng Total RNA)... [Pg.644]


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