Outline of low angle laser light scattering

The attenuated beam is by the condensing lens on the center of the cell. 

The light scattered from the cell is effectively selected by an annular ring at a low angle around 5°C and condensed by the relay lens on the aperture and monitored by the photomultiplier. Simultaneously, a fraction of the incident light is split to be received by a reference photomultiplier. 

Is Installed before the sample injector. The solvent is passed through a guard column (GC) Into the main column. The eluate from the column is monitored by three detectors connected in series, a UV monitor (UV), a precision differential reftactometer (RI) and a LALLS photometer (LS). The output of each detector is recorded (Re). 

Since the output of the refractometer, (Output) ] varies with c and dn/dc we get 

Using monomeric 6SA, we can deduce the value of Kj for the experimental conditions applied. 

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