Osteoclasts activity tests

To clarify the mechanism of action of these diterpenoids on bone resorption, their effect on osteoclast-like cell formation was tested according to the method reported by Takahashi et al.[34]. As a result, both compounds dose-dependently inhibited PTH-induced tartrate resistant acid phosphatase-positive MNC formation. Especially, SDC showed complete inhbition at concentration over 0.1 pM. Next, the effect of SDB and SDC on resorbing activity of osteoclasts (pit forming activity of osteoclast-like cells) was assayed according to the method of Tamura et al. [35]. As indicated in Table 8, both compounds inhibited the pit-formation when osteoclasts obtained by the co-culture were placed on dentine slice in the presence of these compounds. Therefore, the inhibitory effect of... 

Nacre has been tested in human, sheep, and rabbit models (Lamghari et al., 2001). In human patients, fresh woven bone bonds itself throughout the nacre implant, augmented by the heightened activities of osteoblasts and osteoclasts (Figure 1.8). While nacre is stably tolerated in vivo, its degradation and resorption is limited and this... 

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