Organic-Chemical and Enzyme-Catalyzed Methods of Synthesis

Combinations of Organic-Chemical and Enzyme-Catalyzed Methods of Synthesis 

Preparations of sLe Determinants by Enzyme-Catalyzed Processes with Regeneration of Cofactors 

An alternative pathway for the synthesis is based on an analogous transfer of Gal from UDP-Gal and pennits the incoiporation of Gal analogs or labeled Gal precursors. In this case, a different approach has been used to coiwert UDP-Glc into UDP-Gal. UDP-Glc reacts with Gal 1-phosphate under catalysis by Gal 1-phospliate uridylyItransferase to fonn UDP-Gal and Glc 1-phosphate. The Gal 

1-phosphate for this reaction is formed together with ADP from Gal and ATP under catalysis by galactokinase. The byproduct ADP accepts a phosphate group from enolpyruvate phosphate under catalysis by pyruvate kinase. Completing the cycle, the byproduct Glc 1 -phosphate reacts with UTP under catalysis by UDP-Glc pyrophosphors lase to regenerate UDP-Glc. 

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