Tissue organ, defined culture medium

Cells freshly extracted and isolated from an organ or tissue and plated in a defined culture medium (e.g., PBS or L-15 media). During this procedure two parallel processes occur 1) differentiated cells of the original tissue explants usually do not divide and, with time, will successively lose some of their specialized functions (dedifferentiation) and 2) decrease of number of specialized cells (e.g., fibroblasts divide rapidly, and will eventually outnumber the specialized cells). Volume 1(14). 

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