Optimal cutting temperature OCT

Attach the tissues to the sample holder with small amount of optimal cutting temperature (OCT) compounds (Cryo-OCT Compound, Pittsburgh, PA, USA) at the back of tissue. No OCT compounds were used on the tissue area to be cut (see Note 2). 

Optimal Cutting Temperature (OCT) compound (Ted Pella Inc., Redding, CA, USA). 

The use of a cryopreservative solution containing polymer compounds such as a solution with an optimal cutting temperature (OCT) polymer should be restricted to the attachment of... 

Embedding medium for frozen tissue specimens to ensure optimal cutting temperature (OCT, Sakura Finetek, Torrance, CA, USA). 

Gelatin produces less interference for mass spectral analysis than optimal cutting temperature (OCT) media that contains a high concentration of polyethylene glycol (PEG). Keep the gelatin warm before submerging the tissue, otherwise it will become sticky. 

OCT (optimal cutting temperature) compound (Tissue-Tek, ProSciTech, Thuiingowa). 

Cryostats are normally used as a standard tool for slicing frozen tissue to reducing sample contamination. Contamination of the tissue surface due to the use of OCT (optimal cutting temperature polymer) as an embedding medium for stabilizing the organ specimens should be avoided because it would lead to ionization suppression in MALDI-MS analysis. 

Sampling The infected intra-cerebrally mice for Obihiro strain (3) and hamsters for Sc237 (4) at each infected stages were sent from PDRC/NAHI (Ibaraki, Japan) after all the safety precautions for the study were fulfilled. Brain tissues (hippocampus domain) were sampled under P2 level conditions and were embedded adjacently with normal brain tissues (hippocampus domain) into an embedding medium for frozen tissue specimens (OCT Optimal Cutting Temperature Tissue-Tek, Sakura Fine-technical Co., Ltd., Tokyo, Japan), respectively. 

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