Optical robustness protein

Due to the ease of preparation and the chemical as well as optical robustness protein and DNA coated clusters are useful tools in various assays and biochips. The degree to which biological activity is lost depends upon the structural integrity and will vary from protein to protein. A conformational change induced by tight interaction with the metal... 

Yeung et al. addressed the question of whether or not pull samples gave as good equations as samples measured on-line.47 The so-called add-back and process-stream samples were compared for statistical robustness in the equations they produced and their performance in predicting values in an on-line reaction. Materials such as biomass (optical density), protein, and RNA were measured by both techniques. 

There is a strong limitation in the concentration range due to the logarithmic relationship between transmission and concentration (optical densities reasonably to measure range from 0.1 to 1.5). Nevertheless, protein quantification by direct UV-measurement or after staining with dyes in the visible range is a very robust method and can be found, e.g., as a common detection mode in HPLC or other chromatographic techniques. 

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