Operation of the Sequenator

Reagent 1 5% (v/v) solution of PITC in heptane Reagent 2 1.0 M Quadrol-trifluoroacetic acid buffer in n-propanol water (3 4, v/v) pH 9.0 

Reagent 3 anhydrous n-heptafluorobutyric acid Solvent 1 benzene 

Solvent 2 ethyl acetate containing 0.1% (v/v) acetic acid Solvents 1-butyl chloride 

V (vacuum) and w (waste). The duration of stages refers to a reaction temperatur of 50 °C  

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