Online hydrogen generators

UHP Zero Air and Hydrogen Generators for Fuel Gas, 2006 , p. 3. 

On the other hand, several oxidases are known to generate hydrogen peroxide, acting as an oxidant in the CL system, from corresponding substrates. IMERs in which the oxidases are immobilized on adequate supporting materials such as glass beads have been developed. IMERs are often used for flow injection with CL detection of uric acid and glucose, and are also applicable to the CL determination of acetylcholine, choline, polyamines, enzyme substrates, etc., after online HPLC separation. 

Another approach to dealing with the nonelectrochemically active nature of most amino acids is to generate, in situ, chemical reactions at the electrode surfaces to produce electrochem-ically active products for detection. Related to this concept, is the online use of immobilized enzymes (142) to react with amino acids. A by-product of this reaction is hydrogen peroxide, which is then quantified by amperometric detection. 

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