One step purification of an integral membrane protein

A more detailed description of this work, including results for size and charge homogeneity, can be found in Application Note 18-1128-92. 

Histidine-tagged cytochrome bo 3 ubiquinol oxidase from E. coli. 

Integral membrane proteins require the use of detergents for extraction. The concentration and type of detergent that is suitable for a particular extraction must be tested for each situation. 

Frozen cells were mixed with 200 mM Tris-HCl, pH 8.8, 20 mM Na2 -EDTA, 

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