Oligosaccharide synthesis chemoenzymatic approach

Strategies for Synthesis of an Oligosaccharide Library Using a Chemoenzymatic Approach... 

Cyclodextrins (CDs) have the ability to include the hydrophobic area of a compound, and the formed complex becomes soluble in water due to hydroxyl groups orientated on the surface of CDs. Thus, it was proposed that CDs would have similar properties as surfactants in enzymatic glycosylations. Nishimura etuL studied the utility of simple CDs for the synthesis of neoglycolipids using glycosyltransferase. In this approach, the authors attempted to use cell-media synthesis, which was named cellular-chemoenzymatic synthesis, as a triple-factor combination method, to construct oligosaccharide easily. Three types of CD, a-CD, /3-CD, and... 

In the last years, chemoenzymatic and automated approaches have been shown to be useful for the synthesis of oligosaccharides corresponding to regular regions of GAG. The advancement in the knowledge of structure... 

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