Oligosaccharide multi-enzyme systems

G. F. Herrmann, Y. Ichikawa, C. Wandtey, F. C. A. Gaeta, J. C. Paulson, and C.-H. Wong, A new multi-enzyme system for a one-pot synthesis of sialyl oligosaccharides Combined use of P-galactosidase and a(2,6)-sialyltransferase coupled with regeneration in situ of CMP-sialic acid, Tetrahedron Lett. 34 3091 (1993). 

There have also been a limited number of reports showing that slightly altered natural sugars can be introduced into such cyclic multi-enzyme systems to generate non-natural oligosaccharides. Carbohydrates with major non-natural elements will, however, still have to be prepared chemically in the near future. 

Oligosaccharide structures produced by a given cell type will reflect the constitution of the multi-galactosyltransferase system [22] and hence the highly-ordered sequential action of the galactosyltransferase enzymes [23]. Synthesis of oligosaccharides can therefore be seen to depend upon a variety of factors [24] ... 

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