Oligo thiolate

Fluorescent probes containing sulfhydryl-reactive groups can be coupled to DNA molecules containing thiol modification sites. The chemical derivatization methods outlined in Section 2.2 (this chapter) may be used to thiolate the oligo for subsequent modification with a fluorophore. Appropriate fluorescent compounds and their reaction conditions may be found in Chapter 9. The protocol discussed in the previous section can be used as a general guide for labeling DNA molecules. 

In a different type of experiment, Cai et al. [63] measured the induction of SB induced by electrons of 8-68 eV in SAM of oligonucleotides. From their results they extracted effective cross sections and AL for SB. A 50-base long thiolated oligonucleotide (OligoS), 5,-(GCTA)12GC(CH2)3-SS-(CH2)3-OH-3, was labelled at the 5 -end with 32P and chemisorbed at 3 -sulfur(S) end onto a gold... 

Hacker, C., Batteas, J. D., Garno, J. C. et al. 2004 Structural and chemical characterization of mono-fluoro substituted oligo(phenylene-ethynylene) thiolate self-assembled monolayers on gold. Langmuir 20 6195-6205. 

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