O-isobutyl MPA

TABLE 7.3. Characteristics of the procedures for determination of O-isobutyl MPA as a marker of exposure to RVX, in urine and blood plasma... 

FIGURE 7.2. Concentration of O-isobutyl MPA in blood plasma versus time after intramuscular injection of RVX in rats at a dose of 0.8 x LD50. 

Procedures for identification and quantitative assessment of O-isobutyl MPA (a marker of exposure to RVX) in urine and blood plasma have been developed, and toxicokinetic experiments conducted to measure O-alkyl MPA in rat plasma after intramuscular injection of RVX at a dose of 0.8 X LD50. In addition, we have obtained a mass spectrum of human albumin after in vitro incubation of blood serum with RVX. Incubation of commercially available human albumin with RVX with subsequent trypsinolysis and MS/ MS analysis has revealed two sites of binding RVX to human albumin, Tyr 411 and Tyr 150. These data could contribute to development of sensitive and specific diagnostic methods. 

TABLE 10.3 Characteristics of the Procedures for Determination of O-isobutyl MPA as a Marker of Exposure to RVX, in Urine and Blood Plasma... 

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