O’Farrell technique

Johansson, K.-E. (1989a) Protein mapping by two-dimensional polyacrylamide gel electrophoresis (O Farrell technique), in Protein Purification - Principles, I ligh Resolution Methods and Applications (Janson, J.-C. Ryden, L Eds.). VCH, Weinheim. 

Purification of the maize carboxylase, enzymatic assays, preparation of the small subunit as well as cleavage and sequencing of this polypeptide were performed as described recently (11). The components of the heterogeneous moiety of the small subunit of the maize carboxylase were resolved by two-dimensional gel electrophoresis using the O Farrell technique (12). Circular dichroism spectra of the spinach and maize Rubisco were recorded using a Jasco J-20 spectropolarimeter. Small angle X-ray scattering of the maize enzyme was measured as reported in (3,4). 

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