Nucleotides osmium tetroxide

CCM (F1) is based upon a similar principle but uses hydroxylamine and osmium tetroxide to distinguish between mismatched C or T nucleotides, respectively. The position of the mismatch (e.g., the mutation) is defined by sizing on gel electrophoresis after a chemical-mediated cleavage at the reactive position by piperidine. 

K. Burton, Biochem. J., 104, 686 (1967). Oxidation of Pyrimidine Nucleosides and Nucleotides by Osmium Tetroxide. 

ODS octyldecasilene 0s04 osmium tetroxide pdJ new nucleotide in T. brucei PEI polyethyleneimine Pi inorganic orthophosphate PPi pyrophosphate PUR purine PYR pyrimidine... 

FIGURE 1.8 Some chemical reactions of bases and nucleotides, (a) Hydrolysis of a dinucleotide by alkali. Bases are denoted by B1 and B2. R denotes ribose or deoxyribose. (b to k) Chemical reactions (b) bisulfite, (c) chloroacetaldehyde, (d) diethyl pyrocarbonate, (e) dimethyl sulfate, (f) formaldehyde, (g) glyoxal, (h) hydrazine, (i) nitric acid, (j) nitrous acid, and (k) osmium tetroxide. Partly from R. L. P. Adams, J. P. Knowler, and D. P. Leader (1986). In The Biochemistry of the Nucleic Acids, 10th ed., pp. 5—34. Chapman Hall, with permission. 

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