Nucleotides, Nucleosides, and Related Analytes

The levels of 13 urinary nucleosides (e.g., dihydrouridine, pseudouridine, 1-methyladenosine, inosine, guanosine, 5 -deoxy-5 -methylthioadenosine) were monitored at 260 nm and 280 nm using a 30°C column and a 40-min 100/0 40/60 

Micheli and Sestini [439] report on the study of NAD/NAPD precursors, metabolites, and catabolites (e.g., nicotinate and its mononucleotide, nicotinamide and its mononucleotide, AMP, inosine monophosphate) in oythrocytes. Samples were extracted fixim red blood cells and analyzed on a C g column (A — 260 nm and 280 nm). A 93/7 (hold 5 min) — 70/30 (at 6 min hoM 4 min) water (0.1 M KH2PO4 buffer at pH 5.5 with 8mM tetrabutylarmnonium sulfate)/(70/30 water [0.1 M KH2PO4 buffer at pH 5.5 with 8mM tetrabutylarmnonium sulfate]/methanol) gradient generated excellent peak shapes and resolution. Reported erythrocyte levels ranged fix m 10 to 50 nmol/mL. 

Normal and modified urinary nucleosides were monitored on a 30°C C g column (A = 260 nm and 280 nm) using a complex 30-min 100/0 - 40/60 water (25 mM KH2PO4 at pH 4.7)/(60/40 methanol/water) gradient [440]. Out of tire 16 compounds monitored (e.g., uridine, 6-methyladenosine, 5-methyluridine, 2-methyl-guanosine, xanthosine) four compounds (pseudouridine, 1-methyladenosine, 3-methyluridine, 1-methylinosine) were clearly elevated-concentration markers for cancer. Standard concentrations ran fix m 1 to 4pM. Overall resolution was exceptional as were peak shapes. 

The k values for 41 ribonucleotides, deoxynucleotides, cyclic nucleotides, and deoxycyclic nucleotides were generated on a Cjg column (A = 254 nm) using a 4/96 

An excellent separation of five DNA nucleotides (2 -deoxyguanosine, 2 -deoxy-5-methylcytidine, 2 -deoxycytidine, 2 -deoxyadenosine) and their five sulfur mustard adducts (products fixim alleged mustard gas exposure) was generated on a C g column using a complex 40-min 0/100 - 48/52 methanol/water (25 mM ammonium carbonate buffer at pH 8) gradient [442]. Excellent peak shapes and resolution were obtained. 

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