Nucleobases, Nucleosides, and Nucleotides

Brand name Manufacturer Particle size (pm) Pore size (A) Dimensions [length X i.d.] (mm) 

20 (v/v) MeCN/2.5 mmol/L ammonium formate (4) guanine, and (5) cytosine. 

Johnsen et al. separated nucleoside triphosphates on a polymeric zwitterionic phase (ZlC-pHlLlC) that provided a better separation than the respective 

Oxygen was used as the reaction gas in the collision/reaction cell to produce PO by reacting with phosphorus in the gas phase, thereby effectively eliminating the interferences for phosphorus that are normally seen at m/z 31. 

Although HILIC methods offer some promise in potentially reducing ion-suppression effects due to ion-pairing reagents used in conventional separation 

---

The individual nucleobases, nucleosides, and nucleotides all have broad absorption bands with maxima near 260 nm [8], These bands result from overlapping n —> tt ... 

The coordination properties of the nucleobases have been reviewed by Houlton (40) and by Lippert (2). In a recent review, Lippert discussed the influence of the metal coordination on the piSTa of the nucleobases (41), which correlates with their coordination properties. While the coordination properties of nucleobases, nucleosides, and nucleotides have been extensively studied and reviewed, the number of articles dedicated to the coordination properties of nucleic acids is signihcantly smaller. DeRose et al. (42) recently published a systematic review of the site-specific interactions between both main group and transition metal ions with a broad range of nucleic acids from 10 bp DNA duplexes to 300 00 nucleotide RNA molecules as well as with some nucleobases, nucleosides, and nucleotides. They focused on results obtained primarily from X-ray crystallographic studies. Egli also presented information on the metal ion coordination to DNA in reviews dedicated to X-ray studies of nucleic acids (43, 44). Sletten and Fr0ystein (45) reviewed NMR studies of the interaction between nucleic acids and several late transition metal ions and Zn. Binding of metal complexes to DNA by n interactions has been reviewed by Dupureur and Barton (46). 

---