Nucleic acids spectrophotometric assay

B 10. What biomolecules would interfere with the measurement of nucleic acids using the spectrophotometric (Am]) assay ... 

Assay of Ribonuclease. Several kinds of assay have been found useful for studying ribonuclease (RNAase). A spectrophotometric assay is based on the empirical, unexplained observation that there is a decrease in optical density at 300 m/ during hydrolysis of RNA. RNA can be precipitated from solutions of its degradation products by uranyl acetate in trichloroacetic acid the soluble nucleotides can be measured spectro-photometrically or chemically. A manometric assay determines the formation of secondary acid groups through release of CO2 from a bicarbonate buffer acid groups have also been determined by titration. Recently cyclic nucleotides have been used as substrates in place of nucleic acid. ... 

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