Nucleic acids silanized

Plus One REPEL-SILANE ES (a solution of 2% w/v of dichloromethyl silane in octamethyl cyclooctasilane) is used to inhibit the sticking of polyacrylamide gels, agarose gels and nucleic acids to glass surfaces and is available commercially (Amersham Biosciences). 

Kumar A, Larsson O, Parodi D, Liang Z (2000) Silanized nucleic acids a general platform for DNA immobilization. Nucleic Acid Res 28 e71... 

Silanized Nucleic Acids a General DNA Immobilization Platform. 54... 

Keywords Microarray Immobilization Surface modification Coating Silanized nucleic acids... 

Fig. 1 Comparison of a conventional DNA-attachment scheme and the silanized nucleic acid strategy. A In conventional deposition methods the slides are universally coated with silane or poly-lysine, then oligonucleotides or cDNA is deposited on the modified surface. The areas on the chip that are not occupied with DNA will have to be blocked in hybridization experiments. B The DNA or oligonucleotides are modified with a silyl group that can directly immobilize the DNA onto an unmodified glass surface...

Fig. 6 Immobilized functional nucleic acids, a Example fabrication of an aptamer biosen- sor surface (i) Attachment of (glycidoxypropyl)trimethoxy silane (GOPS) to glass surface at low pH results in the formation and subsequent cleavage of an epoxide, (ii) The resultant diol is activated with 1,1-carbonyldiimidazole (CDI) to form a carbonylimidazole.

Immobilization on attachment surface - Work on the use of polyacrylamide gel-immobilized microarrays for nucleic acids has been reported by Zlatanova and Mirzabekov [25]. The use of unmodified oligos to fabricate DNA microarrays where acid-washed slides are coated with epoxy silane before probe deposition has also been reported [26]. 

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