Nucleic acids Macromolecules with primary structure

Hydrophobic interaction chromatography (HIC) is a mode of separation in which molecules in a high-salt environment interact hydrophobically with a nonpolar bonded phase. HIC has been predominantly used to analyze proteins, nucleic acids, and other biological macromolecules by a hydrophobic mechanism when maintenanee of the three-dimensional structure is a primary eoneern [1-4]. The main applications of HIC have been in the area of protein purification because the reeovery is frequently quantitative in terms of both mass and biological activity. 

Colburn and Boutwell [206] concluded that at least there does seem to be a positive correlation of tumorigenesis with one or both of the nucleic acids DNA and RNA. Very recently, it was stated that the critical experiments have yet to be done to ascertain which (if any) of the macromolecules to which hydrocarbons (or their expoxides) bind is the primary target that triggers the carcinogenic process [139]. From quantitative structure-activity analysis, Franke [207] suggests that microsomal arylhydrocarbon benzopyrene hydroxylase may be the first step in chemical carcinogenesis of polycyclic hydrocarbons. 

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