Nucleic acids electrochemical aptasensor development

A reusable electrochemical electrode for the use of low-molecular-weight molecules by its aptamers was demonstrated with the development of an electrochemical aptasensor for adenosine monophosphate (Wu et al., 2007). The anti-adenosine aptamer was functionalized at its end with a redox-active ferrocene unit. An electrode was functionalized with nucleic acid complementary to a region of the aptamer, and this was hybridized to the redox-tethered aptamer, giving rise to a redox response. In the presence of adenosine, an adenosine-aptamer complex was formed, resulting in dissociation of the redox-tethered aptamer from the surface and depletion of the redox signal. The extent of the decrease in electrical response was controlled by concentration of the adenosine analyte. 

Indirect amplified electrochemical analysis of aptamer-protein complexes has employed nanoparticles as labels for the development of electrochemical aptasensors. The use of metal nanoparticles as tracers for the analysis of nucleic acid... 

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