Nucleic acid restriction mapping

Choose Nucleic acid tool of the Sequence menu (i.e., SequenceNucleic Acid-Restriction Map) to open a dialog box. 

Fig. 2. (a) Map of pUC19, a commonly used plasmid vector where the numbers correspond to the positions of the various restriction enzyme cuts and (b) nucleic acid composition of pUC19 from position 393 (5 -end) through position 469 (3 -end) (5,7). 

Walfield, A.M., Storb, U., Seising, E., Zentgraf, H. (1980). Comparison of different rearranged immunoglobulin kappa genes of a myeloma by electromicroscopy and restriction mapping of cloned DNA implications for allelic exclusion . Nucleic Acids Res. 8, 4689-4707. 

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