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Nuclease oligo isoadenylate-activated

Oligo-isoadenylate activated nuclease activity of factor F. T I globin mRNA (0.5 M g) incubated (18) 50 min, 300C with 0.5 ixg F (PC-purified) and 0.16 pmoles, iso-A trimers (X) was analyzed by polyacrylamide gel electrophoresis, 7 iirea. Scanning of autoradiography is shown. Arrow shows direction of migration. [Pg.247]

II. OLIGO-ISOADENYLATE SYNTHESIS AND ACTIVATION OP NUCLEASE P A. Oligo-isoadenylate-Synthetase E... [Pg.246]

C. Oligo-isoadenylate Dependent Nuclease Activity of Factor F... [Pg.248]

The nuclease which degrades oligo-isoadenylate copurified with the iso-A activated ribonuclease P over several steps, but was separated on hydroxylapatite. The iso-A degrading enzyme (nuclease D of Figure 1) splits (2 -5 ) P-A- to yield 5 -AMP, and more slowly (3 -5 )-A-pA. It clearly differs from known ribonucleases that are inhibited by 2 -5 dinucleotides (22). [Pg.249]

See also in sourсe #XX -- [ Pg.204 , Pg.246 , Pg.247 , Pg.248 , Pg.250 , Pg.284 , Pg.285 ]



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Nucleases

Oligo

Oligo isoadenylate

Oligo-isoadenylate Dependent Nuclease Activity of Factor

Oligos

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